dab xname kit

DAB Substrate Kit, Peroxidase (HRP), with Nickel, (3,3′-diaminobenzidine)

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The DAB substrate reacts with HRP to yield an insoluble brown-colored product. This kit includes nickel, providing the option of changing the reaction product from brown to gray-black.


  • Ideal for IHC/ICC/ISH and blots
  • Heat Stable
  • Permanent mounting
  • Ideal for single and multiple labeling
  • No wait times for mixing and dissolving powders or tablets
  • Sufficient reagents to produce 300 ml of working solution


More Information
Unit Size 1 kit
Applications Immunohistochemistry / Immunocytochemistry, In situ hybridization, Blotting Applications, Elispot
Detection Enzyme(s) Peroxidase
Substrate Reaction Product Precipitating Reaction Product
Chromogen Color Brown, Gray-Black
Microscopy Brightfield, Darkfield, Electron, Spectral Imaging
Additional Substrate Properties Heat Resistant, Multiple Labeling
Mounting Aqueous (Hardening), Non-Aqueous (Permanent)
Safety Title WARNING: Cancer –

Kit Components

Kit Contents:

  • 6 ml DAB Reagent 1
  • 6 ml DAB Reagent 2
  • 6 ml DAB Reagent 3
  • 6 ml DAB Reagent 4 (Nickel Optional)


  • IHC Multiplexing Guide
  • Enzyme Substrates Brochure
  • Enzyme Substrate Combinations Chart
  • Counterstain/Substrate Compatibility Chart
  • User Guide
  • Safety Data Sheet
  • Datasheet

Product FAQs

I am performing IHC on tissue sections using a mouse IgG primary antibody. My detection system is a biotinylated anti-mouse IgG secondary antibody, a VECTASTAIN ® Elite ABC peroxidase kit (PK-6100), in combination with a DAB substrate kit (SK-4100), and I do not see any staining. How do I know if the end detection reagents are working?

1 mL) of this DAB substrate into a clean glass test tube. To this 1 ml aliquot, add one drop (

50 µL) of Reagent B only from the VECTASTAIN ® Elite ABC kit. If the HRP enzyme is active and the DAB reacts with it, an immediate color change will be observed. This indicates the end detection reagents are working appropriately.

DAB (3, 3 -diaminobenzidine) HRP substrate produces a dark brown reaction product and can be used for both immunohistochemical and blotting applications. ]]>